Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins

@inproceedings{Arunkumar2012ImmunoprotectionIS,
  title={Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins},
  author={Selvarayar Arunkumar},
  booktitle={Veterinary research forum : an international quarterly journal},
  year={2012}
}
Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a single reactive band at 66 kDa. In immunization trial, sheep were administered intramuscularly with 500… CONTINUE READING
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