Immunoprecipitation techniques for the analysis of transcription factor complexes.

@article{Klenova2002ImmunoprecipitationTF,
  title={Immunoprecipitation techniques for the analysis of transcription factor complexes.},
  author={Elena M. Klenova and Igor Chernukhin and Toshiaki Inoue and Shaharum Shamsuddin and John G Norton},
  journal={Methods},
  year={2002},
  volume={26 3},
  pages={254-9}
}
Interactions among transcription factors can be detected and analyzed by a variety of in vitro and in vivo approaches. In many studies, the existence of putative interactions among transcription factor partners is initially established from yeast two-hybrid screening and in vitro protein association analysis. The ability to detect candidate interacting proteins in coimmunoprecipitates from cell lysates provides an important criterion for establishing the authenticity of such protein… CONTINUE READING

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