Immunoperoxidase and enzyme-histochemical demonstration of human skin tryptase in cutaneous mast cells in normal and mastocytoma skin

@article{Harvima1988ImmunoperoxidaseAE,
  title={Immunoperoxidase and enzyme-histochemical demonstration of human skin tryptase in cutaneous mast cells in normal and mastocytoma skin},
  author={Ilkka Tapani Harvima and Anita Naukkarinen and Rauno J. Harvima and Jorma E. Fr{\"a}ki},
  journal={Archives of Dermatological Research},
  year={1988},
  volume={280},
  pages={363-370}
}
Trypsin-like proteinase isolated from human skin was localized in cutaneous mast cells using immunoperoxidase and enzyme-histochemical techniques. Skin biopsy specimens were taken from four mastocytoma and four healthy patients. Immunoperoxidase staining was performed with protein A-sepharose purified rabbit polyclonal antibody raised against human skin tryptase and using aminoethylcarbazole as chromogen. The positively stained cells in the dermis were granular in character. Using peptide 4… CONTINUE READING