Immunomodulating Activity of Agaricus brasiliensis KA21 in Mice and in Human Volunteers

  title={Immunomodulating Activity of Agaricus brasiliensis KA21 in Mice and in Human Volunteers},
  author={Ying Liu and Yasushi Fukuwatari and Ko Okumura and Kazuyoshi Takeda and Ken-Ichi Ishibashi and Mai Furukawa and Naohito Ohno and Kazu Mori and Ming Gao and Masuro Motoi},
  journal={Evidence-based Complementary and Alternative Medicine : eCAM},
  pages={205 - 219}
We performed studies on murine models and human volunteers to examine the immunoenhancing effects of the naturally outdoor-cultivated fruit body of Agaricus brasiliensis KA21 (i.e. Agaricus blazei). Antitumor, leukocyte-enhancing, hepatopathy-alleviating and endotoxin shock-alleviating effects were found in mice. In the human study, percentage body fat, percentage visceral fat, blood cholesterol level and blood glucose level were decreased, and natural killer cell activity was increased. Taken… 
Effects of Agaricus brasiliensis mushroom in Walker-256 tumor-bearing rats.
The beneficial effects of A. brasiliensis in tumor treatment, mainly related to cachexia, can be partly related to antioxidant activity and to reduction of weight loss and tumor growth.
Immunostimulation-Mediated Anti-tumor Activity of Bamboo (Sasa senanensis) Leaf Extracts Obtained Under ‘Vigorous’ Condition
Activation of macrophages and NK cells by the extracts suggests that the anti-tumor efficacy of the extract is mediated by immunopotentiation, and warrants further translation and clinical investigations.
Agaricus brasiliensis-enriched functional product promotes in mice increase in HDL levels and immunomodulate to Th1 CD4+T subsets. A. brasiliensis functional product and biological benefits.
The solid state culture demonstrated to be a very good technique to develop A. brasiliensis mycelium on wheat grains to obtain a protein enriched biocultured material and promoted an increase in the “good cholesterol” plasma concentration and probably expansion of the Th1 CD4+T cells.
Effect of Agaricus brasiliensis-derived cold water extract on Toll-like receptor 2-dependent cytokine production in vitro
Cold water extract of the fruit body of A. brasiliensis is prepared and fruit body has some water-soluble TLR ligand complexes, and TLR2 on splenocytes strongly induces IL-6 production, which is suggested to be the receptor that modulates IL- 6 production.
Agaricus brasiliensis mycelium supplementation in Sarcoma 180tumour-bearing mice reverses the immune response induced by the tumour
It is confirmed that dietary supplementation with Agaricus may offer immuno-modulatory activity and protection against tumour growth and downregulated Tumor necrosis factor alpha production in plasma.
Evaluation of Agaricus blazei in vivo for antigenotoxic, anticarcinogenic, phagocytic and immunomodulatory activities.
Effect of mushroom Agaricus blazei on immune response and development of experimental cerebral malaria
This study strongly suggests that the administration of A. blazei (aqueous extract or fraction C) was effective in improving the consequences of CM in mice and may provide novel therapeutic strategies.
Ganoderma lucidum and Auricularia polytricha Mushrooms Protect against Carbofuran-Induced Toxicity in Rats
The coadministration of mushroom extracts significantly ameliorated the toxic effects of the CF and the GL mushroom extract was more efficacious than that of the AP mushroom, possibly due to the presence of high levels of phenolic compounds and other antioxidants in theGL mushroom.


Effects of extracts from Brazilian sun-mushroom (Agaricus blazei) on the NK activity and lymphoproliferative responsiveness of Ehrlich tumor-bearing mice.
In Vitro Augmentation of Natural Killer Activity and Interferon-γ Production in Murine Spleen Cells with Agaricus blazei Fruiting Body Fractions
Aqueous extracts of the Agaricus blazei fruiting body prepared at different temperatures were fractionated by ethanol precipitation with various ethanol concentrations, showing the strongest augmenting effect on interferon (IFN)-γ production and IFN-γ production by fraction A-50.
Suppressing effects of daily oral supplementation of beta-glucan extracted from Agaricus blazei Murill on spontaneous and peritoneal disseminated metastasis in mouse model
It is shown that beta-glucan had cytotoxic effect against human ovarian cancer HRA cells, but not against murine Lewis lung cancer 3LL cells, in vitro, and treatment with beta- glucan may be beneficial for cancer patients with or at risk for metastasis.
Effect of Maitake (Grifola frondosa) D-Fraction on the activation of NK cells in cancer patients.
Maitake D-Fraction appears to repress cancer progression and primarily exerts its effect through stimulation of NK activity, and measurement of NK cell activity is concluded to be a useful clinical parameter in monitoring disease progression during and following immunotherapy with maitake LFraction.
The use of mushroom glucans and proteoglycans in cancer treatment.
  • P. Kidd
  • Medicine, Biology
    Alternative medicine review : a journal of clinical therapeutic
  • 2000
Two proteoglycans from Coriolus versicolor - PSK and PSP - have demonstrated the most promise and their extremely high tolerability, proven benefits to survival and quality of life, and compatibility with chemotherapy and radiation therapy makes them well suited for cancer management regimens.
Natural killer cell activity and quality of life were improved by consumption of a mushroom extract, Agaricus blazei Murill Kyowa, in gynecological cancer patients undergoing chemotherapy.
  • W. Ahn, D. Kim, I. Lee
  • Medicine
    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
  • 2004
ABMK treatment might be beneficial for gynecological cancer patients undergoing chemotherapy, and chemotherapy-associated side effects such as appetite, alopecia, emotional stability, and general weakness were all improved by ABMK treatment.
IL-12 Production Induced by Agaricus blazei Fraction H (ABH) Involves Toll-like Receptor (TLR)
  • H. Kasai, L. He, M. Ito
  • Biology
    Evidence-based complementary and alternative medicine : eCAM
  • 2004
Findings suggest that ABH activated Mo/Mφ in a manner dependent on CD14/TLR4 and NK activity, which suggests that oral administration of ABH enhanced natural killer activity in the spleen.
Lack of carcinogenicity of agaritine by subcutaneous administration in mice
The field is discussed in the light of the obtained results, since some of the breakdown products of A were shown to be carcinogenic in mice and the mushroom itself was found to be mutagenic.