Immunomagnetic tumor cell enrichment is promising in detecting circulating breast cancer cells.

Abstract

OBJECTIVE Magnetic-activated cell separation (MACS) for the enrichment of tumor cells was evaluated with immunocytochemistry (ICC) and flow cytometry (FCM). METHODS Blood (20 ml) was sampled in 36 affected patients before surgery. Nucleated blood cells were obtained with the removal of red blood cells in the buffy coat. Nucleated blood cells (2 x 10(6… (More)

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