Immunomagnetic separation and solid-phase detection of Bordetella pertussis.

@article{Stark1996ImmunomagneticSA,
  title={Immunomagnetic separation and solid-phase detection of Bordetella pertussis.},
  author={Michael Stark and Elisabet Reizenstein and Mathias Uhl{\'e}n and Joakim Lundeberg},
  journal={Journal of clinical microbiology},
  year={1996},
  volume={34 4},
  pages={
          778-84
        }
}
In the present study, novel solid-phase methods were used for both sample preparation and PCR detection of Bordetella pertussis. The sample preparation was performed by immunomagnetic separation with paramagnetic beads coated with polyclonal antibodies directed toward the surface antigens of the bacteria. The precoated immunobeads were directly used on nasopharyngeal aspirates to capture the bacteria on the solid support and were subsequently transferred to the PCR tube with no further… CONTINUE READING

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