Immunomagnetic separation and quantification of butyrylcholinesterase nerve agent adducts in human serum.

@article{Sporty2010ImmunomagneticSA,
  title={Immunomagnetic separation and quantification of butyrylcholinesterase nerve agent adducts in human serum.},
  author={Jennifer L S Sporty and Sharon Warford Lemire and E. Michael Jakubowski and Julie A Renner and Ronald T. Evans and Robert F. Williams and Jurgen G Schmidt and Marcel J. van der Schans and Daan Noort and Rudolph C. Johnson},
  journal={Analytical chemistry},
  year={2010},
  volume={82 15},
  pages={
          6593-600
        }
}
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum samples. The particle-antibody-BuChE product was rinsed and directly digested with pepsin. Native and isotopically enriched nonapeptides corresponding to the pepsin digest products for uninhibited… CONTINUE READING
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