Immunomagnetic purification of fluorescent Legionella-containing vacuoles.


Protozoa are natural reservoirs of the environmental bacterium Legionella pneumophila. Upon inhalation of Legionella-laden aerosols, the amoeba-resistant bacteria replicate within human alveolar macrophages causing the severe pneumonia "Legionnaires' disease." Within host cells, including Dictyostelium discoideum, L. pneumophila establishes a custom… (More)
DOI: 10.1007/978-1-62703-302-2_24


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