Immunological studies of yeast nuclear RNA polymerases at the subunit level.

  title={Immunological studies of yeast nuclear RNA polymerases at the subunit level.},
  author={J. M. Buhler and Janine Huet and K. E. Davies and A. Sentenac and Pierre Fromageot},
  journal={The Journal of biological chemistry},
  volume={255 20},
Antisera were raised against native RNA polymerases A or B, as well as against each individual subunit of RNA polymerase A from the yeast Saccharmoyces cerevisiae. The affinity spectrum of antibodies was evaluated by reacting electrophoretically separated enzyme subunits, transferred to a membrane, with 125I-labeled immunoglobulins. Alternatively, the subunit . immunoglobulin complex was revealed by 125I-labeled Protein A. Antibodies directed against native RNA polymerase A recognized the… CONTINUE READING

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