Immunological identification of vesicular nucleotide transporter in intestinal L cells.

  title={Immunological identification of vesicular nucleotide transporter in intestinal L cells.},
  author={Yuika Harada and Miki Hiasa},
  journal={Biological \& pharmaceutical bulletin},
  volume={37 7},
It is well established that vesicular nucleotide transporter (VNUT) is responsible for vesicular storage of nucleotides such as ATP, and that VNUT-expressing cells can secrete nucleotides upon exocytosis, playing an important role in purinergic chemical transmission. In the present study, we show that VNUT is expressed in intestinal L cells. Immunohistochemical evidence indicated that VNUT is present in glucagon-like peptide 1 (GLP-1) containing cells in rat intestine. VNUT immunoreactivity is… Expand
Vesicular nucleotide transporter (VNUT): appearance of an actress on the stage of purinergic signaling
Mice with knockout of the VNUT gene lose vesicular storage and release of ATP from neurons and neuroendocrine cells, resulting in blockage of the initiation of purinergic chemical transmission. Expand
Vesicular nucleotide transporter mediates ATP release and migration in neutrophils
It is suggested that tertiary granule-localized VNUT is responsible for vesicular ATP release and subsequent neutrophil migration, and an additional mechanism through which ATP is released by neutrophils is suggested. Expand
Expression of Vesicular Nucleotide Transporter in the Mouse Retina.
Results indicate that functional VNUT is expressed in the rodent retina and suggest that ATP is released from photoreceptor cells, bipolar cells, amacrine cells, and astrocytes as well as Müller cells to initiate purinergic chemical transmission. Expand
Impairment of vesicular ATP release affects glucose metabolism and increases insulin sensitivity
It is suggested that vesicular ATP and/or its degradation products act as feedback regulators in catecholamine and insulin secretion, thereby regulating blood glucose homeostasis and improving glucose tolerance and low blood glucose upon fasting due to increased insulin sensitivity in Vnut−/− mice. Expand
Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain
Data suggest DA neurons co-release ATP via calcium dependent exocytosis and in the retina this may modulate the visual response by activating purine receptors on closely associated neurons. Expand
Regulation of Glucagon-like Peptide-1 Exocytosis from the Primary L-cell by the SNARE Protein Syntaxin-1a
Glucagon-like peptide-1 (GLP-1) is an intestinal L-cell hormone that potentiates glucosedependent insulin secretion. To develop novel approaches for the treatment of type 2 diabetes, the capacity toExpand
Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons
It is demonstrated that GLP-1 secreting cells release ATP and that this stimulates nodose neurons and enterocytes in a paracrine manner in vitro, and that ATP acts as an additional signal triggering vagal activation and potentially synergising with the actions of locally elevated peptide hormone concentrations. Expand
VNUT/SLC17A9, a vesicular nucleotide transporter, regulates osteoblast differentiation
It is demonstrated that VnUT is expressed in osteoblasts and that VNUT inhibits osteoblast differentiation in response to compressive force by mechanisms related to ATP release and P2X7R and/or P2Y2R activity. Expand
Fluorescent Labeling and Quantification of Vesicular ATP Release Using Live Cell Imaging.
Methods are described for fluorescent labeling of VNUT positive cells and quantification of vesicular ATP release using live cell imaging, useful for unveiling the intricacies of purinergic neurotransmission. Expand
Novel modes of communication between neuronal activity and microglial process dynamics
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Identification of a vesicular nucleotide transporter
Findings strongly suggest that SLC17A9 protein is a vesicular nucleotide transporter and should lead to the elucidation of the molecular mechanism of ATP secretion in purinergic signal transmission. Expand
Vesicular nucleotide transporter is involved in ATP storage of secretory lysosomes in astrocytes.
It is shown by immunocytochemical analysis under confocal microscope and live cell imaging under total internal reflection fluorescence microscope that lysosome-associated VNUT is responsible for ATP release in astrocytes, and the participation of VN UT in ATP storage in secretory lysoomes during lysOSomal exocytosis of ATP from astroCytes is indicated. Expand
Vesicular nucleotide transporter regulates the nucleotide content in airway epithelial mucin granules.
Results suggest that VNUT is the relevant nucleotide transporter responsible for the uptake of cytosolic nucleotides into mucin granules and contributes to the release of purinergic signaling molecules necessary for the proper hydration of co-released mucins. Expand
Vesicular storage and secretion of l‐glutamate from glucagon‐like peptide 1‐secreting clonal intestinal L cells
The results demonstrated that GLUTag cells stored l‐glutamate in secretory granules and secreted it with GLP‐1 by exocytosis, which support the concept of l‐ glutamate‐mediated intercellular signaling in the vicinity of intestinal L cells. Expand
Vesicular nucleotide transporter-mediated ATP release regulates insulin secretion.
This study reveals VNUT is expressed in pancreatic β cells and plays an essential and novel role in regulating insulin secretion through vesicular ATP release and extracellular purinergic signaling. Expand
Regulation of Purinergic Signaling in Biliary Epithelial Cells by Exocytosis of SLC17A9-dependent ATP-enriched Vesicles*
The findings are consistent with the existence of an SLC17A9-dependent ATP-enriched vesicular pool in biliary epithelium that undergoes regulated exocytosis to initiate purinergic signaling. Expand
Functional and anatomical identification of a vesicular transporter mediating neuronal ATP release.
It is demonstrated that ATP release from cultured rat hippocampal neurons is sensitive to RNAi-mediated knockdown of the recently identified vesicular nucleotide transporter (VNUT or SLC17A9), which mediates transport of ATP into synaptic vesicles of hippocampusal neurons, thereby conferring a purinergic phenotype to these cells. Expand
Secretory Granule-mediated Co-secretion ofl-Glutamate and Glucagon Triggers Glutamatergic Signal Transmission in Islets of Langerhans*
Results indicate that co-secretion ofl-glutamate and glucagon from α cells under low glucose conditions triggers GABA secretion from β cells and defines the mode of action of l- glutamate as a regulatory molecule for the endocrine function. Expand
Vesicular nucleotide transport: a brief history and the vesicular nucleotide transporter as a target for drug development.
The history of vesicular storage of nucleotides and the identification of the vESicular nucleotide transporter (VNUT) responsible for the process are summarized and the significance of VNUT as a drug target to control purinergic chemical transmission is discussed. Expand
Activation of proglucagon gene transcription by protein kinase-A in a novel mouse enteroendocrine cell line.
Observations provide evidence for the direct induction of proglucagon gene transcription by a cAMP-dependent pathway and suggest that the GLUTag cell line represents a useful model for the analysis of the molecular determinants of enteroendocrine gene expression. Expand