Immunological identification of vesicular nucleotide transporter in intestinal L cells.

@article{Harada2014ImmunologicalIO,
  title={Immunological identification of vesicular nucleotide transporter in intestinal L cells.},
  author={Yuika Harada and Miki Hiasa},
  journal={Biological \& pharmaceutical bulletin},
  year={2014},
  volume={37 7},
  pages={
          1090-5
        }
}
It is well established that vesicular nucleotide transporter (VNUT) is responsible for vesicular storage of nucleotides such as ATP, and that VNUT-expressing cells can secrete nucleotides upon exocytosis, playing an important role in purinergic chemical transmission. In the present study, we show that VNUT is expressed in intestinal L cells. Immunohistochemical evidence indicated that VNUT is present in glucagon-like peptide 1 (GLP-1) containing cells in rat intestine. VNUT immunoreactivity is… Expand
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Novel modes of communication between neuronal activity and microglial process dynamics
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References

SHOWING 1-10 OF 22 REFERENCES
Identification of a vesicular nucleotide transporter
TLDR
Findings strongly suggest that SLC17A9 protein is a vesicular nucleotide transporter and should lead to the elucidation of the molecular mechanism of ATP secretion in purinergic signal transmission. Expand
Vesicular nucleotide transporter is involved in ATP storage of secretory lysosomes in astrocytes.
TLDR
It is shown by immunocytochemical analysis under confocal microscope and live cell imaging under total internal reflection fluorescence microscope that lysosome-associated VNUT is responsible for ATP release in astrocytes, and the participation of VN UT in ATP storage in secretory lysoomes during lysOSomal exocytosis of ATP from astroCytes is indicated. Expand
Vesicular nucleotide transporter regulates the nucleotide content in airway epithelial mucin granules.
TLDR
Results suggest that VNUT is the relevant nucleotide transporter responsible for the uptake of cytosolic nucleotides into mucin granules and contributes to the release of purinergic signaling molecules necessary for the proper hydration of co-released mucins. Expand
Vesicular storage and secretion of l‐glutamate from glucagon‐like peptide 1‐secreting clonal intestinal L cells
TLDR
The results demonstrated that GLUTag cells stored l‐glutamate in secretory granules and secreted it with GLP‐1 by exocytosis, which support the concept of l‐ glutamate‐mediated intercellular signaling in the vicinity of intestinal L cells. Expand
Vesicular nucleotide transporter-mediated ATP release regulates insulin secretion.
TLDR
This study reveals VNUT is expressed in pancreatic β cells and plays an essential and novel role in regulating insulin secretion through vesicular ATP release and extracellular purinergic signaling. Expand
Regulation of Purinergic Signaling in Biliary Epithelial Cells by Exocytosis of SLC17A9-dependent ATP-enriched Vesicles*
TLDR
The findings are consistent with the existence of an SLC17A9-dependent ATP-enriched vesicular pool in biliary epithelium that undergoes regulated exocytosis to initiate purinergic signaling. Expand
Functional and anatomical identification of a vesicular transporter mediating neuronal ATP release.
TLDR
It is demonstrated that ATP release from cultured rat hippocampal neurons is sensitive to RNAi-mediated knockdown of the recently identified vesicular nucleotide transporter (VNUT or SLC17A9), which mediates transport of ATP into synaptic vesicles of hippocampusal neurons, thereby conferring a purinergic phenotype to these cells. Expand
Secretory Granule-mediated Co-secretion ofl-Glutamate and Glucagon Triggers Glutamatergic Signal Transmission in Islets of Langerhans*
TLDR
Results indicate that co-secretion ofl-glutamate and glucagon from α cells under low glucose conditions triggers GABA secretion from β cells and defines the mode of action of l- glutamate as a regulatory molecule for the endocrine function. Expand
Vesicular nucleotide transport: a brief history and the vesicular nucleotide transporter as a target for drug development.
TLDR
The history of vesicular storage of nucleotides and the identification of the vESicular nucleotide transporter (VNUT) responsible for the process are summarized and the significance of VNUT as a drug target to control purinergic chemical transmission is discussed. Expand
Activation of proglucagon gene transcription by protein kinase-A in a novel mouse enteroendocrine cell line.
TLDR
Observations provide evidence for the direct induction of proglucagon gene transcription by a cAMP-dependent pathway and suggest that the GLUTag cell line represents a useful model for the analysis of the molecular determinants of enteroendocrine gene expression. Expand
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