Immunological detection of the messenger RNA cap-binding protein.


The 24-kilodalton messenger RNA cap-binding protein (CBP) was purified from the rabbit reticulocyte postribosomal supernatant fraction using an affinity resin consisting of the p-aminophenyl gamma-ester of m7GTP coupled to Sepharose. The affinity-purified CBP was used to raise a goat antiserum. Anti-CBP antibodies were purified by adsorption to CBP coupled… (More)


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@article{Hiremath1985ImmunologicalDO, title={Immunological detection of the messenger RNA cap-binding protein.}, author={Leena S Hiremath and Nancy R. Webb and R. E. Rhoads}, journal={The Journal of biological chemistry}, year={1985}, volume={260 13}, pages={7843-9} }