Immunological comparison of scrapie-associated fibrils isolated from animals infected with four different scrapie strains

@article{Kascsak1986ImmunologicalCO,
  title={Immunological comparison of scrapie-associated fibrils isolated from animals infected with four different scrapie strains},
  author={Richard J. Kascsak and Richard Rubenstein and Patricia A. Merz and Richard I. Carp and Nikolaos K. Robakis and Henryk M. Wiśniewski and Heino Diringer},
  journal={Journal of Virology},
  year={1986},
  volume={59},
  pages={676 - 683}
}
Scrapie-associated fibrils (SAFs) are abnormal filamentous structures that are uniquely associated with unconventional slow virus diseases. The antigenic relationships of SAFs from animals infected with four biologically distinct scrapie strains were investigated by using antisera raised to purified SAF proteins. Rabbit antisera were raised to SAFs isolated from mice infected with the ME7 scrapie strain and to SAFs isolated from hamsters infected with the 263K scrapie strain. A strong antigenic… 
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The fibrils observed in purified preparations share structural and antigenic similarities plus biochemically related peptides with SAF present in non-protease-treated preparations, and are considered to be the same in each preparation.
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TLDR
The multiple forms of PrP found in SAF fractions from mouse brain affected by the ME7 strain of scrapie are characterized and a novel, as yet unidentified, amino acid derivative of the arginine residue at position 3 in both hamster and mouse PrP 33-35 is found, which may predispose PrP to form SAF.
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TLDR
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TLDR
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Immunological analysis of host and agent effects on Creutzfeldt-Jakob disease and scrapie prion proteins
TLDR
Evidence does not support, although it does not exclude, agent-mediated characteristics independent of host-mediated ones for scrapie and CJD, and the profile of immunoreactive proteins for CJD prions isolated from mice, guinea pigs, and humans appeared distinct.
Molecular pathology of scrapie-associated fibril protein (PrP) in mouse brain affected by the ME7 strain of scrapie.
TLDR
The characterisation of the multiple forms of PrP found in SAF fractions purified from mouse brain affected by the ME7 strain of scrapie and a novel, as yet unidentified, amino-acid derivative of the arginine residue at position 3 in mouse PrP is found, which may predispose PrP to form SAF.
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TLDR
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TLDR
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TLDR
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TLDR
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