Immunological characterization and clinical implication of cobalamin binding protein in human gastric cancer.

@article{Wakatsuki1989ImmunologicalCA,
  title={Immunological characterization and clinical implication of cobalamin binding protein in human gastric cancer.},
  author={Y. Wakatsuki and Mitsuo Inada and Hideki Kudo and G Oshio and Takaaki Masuda and Takashi Miyake and Tsunekazu Kita},
  journal={Cancer research},
  year={1989},
  volume={49 11},
  pages={3122-8}
}
Cobalamin (vitamin B12) binding protein was purified from gastric cancer extracts and from serum-free culture medium of cancer cell line KATOH-III. The molecular weight, determined by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was 70,000 and the pI was 2.8 to 3.2. From biochemical and immunological properties, this cobalamin binding protein was considered to be an isoprotein of cobalamin R binder. Monoclonal antibodies were produced against saliva R and… CONTINUE READING
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