Immunological characteristics and localization of the Trichinella spiralis glutathione S-transferase.

@article{Rojas1997ImmunologicalCA,
  title={Immunological characteristics and localization of the Trichinella spiralis glutathione S-transferase.},
  author={Jos{\'e} Mar{\'i}a Rojas and M Rodr{\'i}guez-Osorio and V G{\'o}mez-Garc{\'i}a},
  journal={The Journal of parasitology},
  year={1997},
  volume={83 4},
  pages={630-5}
}
Trichinella spiralis glutathione S-transferase (TsGST) was isolated from crude extracts of L1 larvae by glutathione-affinity chromatography. Two closely migrating polypeptides with molecular masses of 28.5 and 28 kDa were identified by electrophoresis. Three isoforms of pI 5.6, 5.8, and 6.0 were detected by isoelectric focusing. Purified TsGST showed a low transferase activity as measured with 1-chloro-2,4-dinitrobenzene; glutathione peroxidase activity was also demonstrated using cumene… CONTINUE READING