Immunological and biochemical properties of transverse tubule membranes isolated from rabbit skeletal muscle.

@article{Rosemblatt1981ImmunologicalAB,
  title={Immunological and biochemical properties of transverse tubule membranes isolated from rabbit skeletal muscle.},
  author={Mario Rosemblatt and Cecilia Hidalgo and Cecilia Vergara and Noriaki Ikemoto},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 15},
  pages={8140-8}
}
A new method for isolating transverse tubule membranes from rabbit skeletal muscle has been developed. This procedure has the advantage of being mild, fast, and producing with good yields a purified membrane fraction. The transverse tubule membranes are purified by a discontinuous sucrose density centrifugation after loading contaminating light sarcoplasmic reticulum vesicles with calcium phosphate in the presence of ATP. Immunofluorescence staining of cryostat sections of rabbit psoas muscle… CONTINUE READING
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