Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) Serum Soluble CD21 in Health and Disease

Abstract

S AND CONFERENCES 1. Poster presentation in 33 Annual Meeting of the German Society of Immunology Sep 2002, Marburg, Germany. M. Masilamani and H. Illges, Isolation, characterization and quantification of soluble CD21 from human plasma: Is plasma sCD21 an indicator of autoimmune reaction in vivo?, Immunobiology 2002, 206(1-3):228 2. Poster presentation in Joint Annual Meeting of the German Society of Immunology and the Dutch Society of Immunology, Nov 2001, Duesseldorf, Germany. M. Masilamani, E.von Seydlitz and H. Illges, T cell expressed CD21: Downregulation during activation by anti-CD3, anti-CD28 and IL2, Immunobiology 2001, 203(1-3):78 The results of publications 1-4 are presented in this dissertation. CD21 In Health and Disease Abbreviations Univerisität Konstanz vi ABBREVIATIONS Ab, mAb antibody, monoclonal antibody Ag antigen APC antigen present cell ATP adenosine-triphosphate °C degree celcius cDNA complementary DNA CD21, sCD21 cluster of differentiation-21, soluble CD21 CO2 carbondioxide CRI, CRII complement receptor type I, type II CTL cytotoxic T lymphocyte CVID combined variable immune deficiency Da, kDa dalton, kilodalton (molecular weight) DMEM dulbecco ́s modified eagle medium DMSO dimethylsulfoxide DNA deoxy ribonucleic acid EBV Epstein-Barr Virus EDTA ethylenediamine-tetraacetic acid e.g. for example ELISA enzyme-linked immunosorbent assay EtBr ethidium bromide EtOH ethanol F(ab ́)2 divalent antigen-binding fragment FACS fluoresence activated cell sorting FCS fetal calf serum FITC fluorescein isothiocyanate g, mg, μg, ng gram, milligram, microgram, nanogram h hour HIV human immunodeficiency virus IC immune complex IFN interferon IgG immunoglobulin G IgM immunoglobulin M IP immunoprecipitation IL interleukin i.v. intravenous kbp kilo basepair l, ml, μl liter, milliliter, microliter LPS lipopolysachharide CD21 In Health and Disease Abbreviations Universität Konstanz vii m, cm, μm, meter, centimeter, micrometer M, mM, μM molar, millimolar, micromolar MeOH methanol MHC major histocompatibility complex min minute n number in study or group NK natural killer (cell) NP-40 nonidet P-40 O.D optical density PBL peripheral blood lymphocytes PBMC peripheral blood mononuclear cells PBS phosphate buffered saline PCR polymerase chain reaction PE phycoerythrin PHA phytohemagglutinin PKC protein kinase C PMA phorbol 12-myristate 13acetate PWM pokeweed mitogen r recombinant RPMI tissue culture medium (Rosewell Park Memorial Institute) RT-PCR reverse transcriptasepolymerase chain reaction PMN polymorphonuclear cells r recombinant RA rheumatoid arthritis RBC red blood cells RNA ribonucleic acid RPM rounds per minute RT room temperature SDS sodium dodecyl sulfate SDS-PAGE SDS-polyacrylamide gel electrophoresis sec second SLE systemic lupus erythematosus S value Svedberg value TBE Tris borate EDTA buffer TBS Tris buffered saline TCR T cell receptor Th T helper (cell) TNF tumor necrosis factor TNF-R tumor necrosis factor receptor V volt vs. versus WBC white blood cell CD21 In Health and Disease

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@inproceedings{BIOLOGIE2003ImmunologicalIO, title={Immunological Investigation of Human Complement Receptor Type II (CR2/CD21) Serum Soluble CD21 in Health and Disease}, author={FACHBEREICH BIOLOGIE and Madhan Masilamani and Rolf Knippers and Elizabeth Naidoo and Hans-J{\"{u}rgen Apell and Harald Illges and Elizabeth von Seydlitz and Martin Bastmeyer}, year={2003} }