Immunological Detection and Characterization

  title={Immunological Detection and Characterization},
  author={Robert L. Atmar},
  journal={Viral Infections of Humans},
  pages={47 - 62}
  • R. Atmar
  • Published 27 February 2014
  • Medicine
  • Viral Infections of Humans
Immunological methods have been used for viral diagnosis for more than 100 years. Although molecular methods are replacing many older methods of viral diagnosis, there is still a significant role for immunological methods to guide patient care and in the performance of epidemiologic studies. Identification of viral antigens in clinical samples can be accomplished rapidly through the use of point-of-care lateral immunoassays or through the use of more traditional immunofluorescence and enzyme… Expand
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Viral diagnosis by antigen detection techniques.
  • M. Grandien
  • Biology, Medicine
  • Clinical and diagnostic virology
  • 1996
Although, in virology, more sensitive results are obtained by the gene detection method, PCR, directly in clinical samples, viral antigen detection tests are increasingly used because of their low demand on laboratory equipment, their rapid and early result and relatively low cost. Expand
Detection of viral antigens by time-resolved fluoroimmunoassay.
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The findings suggested that the two strains of CHIKV using variants A226 and 226V resulted in variation in sensitivities of the assays evaluated, and demonstrated the importance of appraisal of different diagnostic assays before their application in clinical and operational settings. Expand
Immunoserology of infectious diseases.
  • K. James
  • Biology, Medicine
  • Clinical Microbiology Reviews
  • 1990
Methods for detecting antibody to verify immunity differ from immunologic methods to diagnose disease; techniques to detect soluble antigens present in active infectious states may appear similar to those used to detect antibody, but their differences should be appreciated. Expand
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Lennette's laboratory diagnosis of viral infections
The clinical laboratory in the management of viral infections and the design of molecular virologic tests for nucleic acid amplification products. Expand
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