Immunologic mediator release from rhesus and cynomolgus monkey lung.

  title={Immunologic mediator release from rhesus and cynomolgus monkey lung.},
  author={Barry M. Weichman and Linda S. Hostelley and Sheila P. Bostick and Lawrence William Chakrin},
  journal={Journal of pharmacological methods},
  volume={4 1},
1 Citations



The capacity of human immunoglobulin E to mediate the release of histamine and slow reacting substance of anaphylaxis (SRS-A) from monkey lung.

In the reversed-type reactions, 7S anti-IgE and its F(ab′)2 fragments had comparable activity in mediating the release of both histamine and SRS-A, and Fab′ fragments did not release either mediator suggesting that bridging of two cellbound IgE molecules may be the initial step of the reaction.

Immunologic release of chemical mediators from human tissues.

This review focuses on the pharmacologic modulation of this immunologic modulation as well as the biochemical events comprising the activation and secretory phases of IgE immunoglobulins specifically bound to the cell surface receptors of mast cells.

Passive sensitization of monkey lung fragments with sera of timothy-sensitive patients. I. Spectrofluorometric analysis of histamine release.

It is suggested that the use of adult monkey lung tissue may provide a means to study the mechanism of antibody fixation and the sensitivity of the method described may permit the detection of reagin in the sera of allergic patients not detectable by the P-K test.

Preformed eosinophil chemotactic factor of anaphylaxis (ECF-A).

The sum of the ECF-A released by antigen and that remaining in the sensitized challenged human or guinea pig lung tissue or isolated rat mast cells equalled that extractable from the equivalent unchallenged lung tissue, indicating that the eosinophil chemotactic factor of anaphylaxis available exists entirely in a preformed state.

Slow reacting substance of anaphylaxis.

Animal models of the asthmatic state.

This discussion will include a brief review of the characteristics of asthma, the types of animal models that have evolved with comments on how tQese systems might relate to the human disease state, and summaries of some of the studies that have been carried out using these animal respiratory responses.

A modified fluorometric procedure for tissue histamine and its distribution in various animals.

  • A. AntonD. Sayre
  • Biology, Chemistry
    The Journal of pharmacology and experimental therapeutics
  • 1969
Under the proper conditions of K 2 HPO 4 and pH, histamine is selectively extracted into isoamyl alcohol, and the fluorescence of the fluorophor is maximized in the presence of citric acid.

Pyranenamines: a new series of antiallergic compounds.

A new class of potent, orally active, antiallergic compounds, the 3-[(arylamino)ethylidene]-5-acylpyrantriones, hereafter referred to as pyranenamines, as evaluated not only in the traditional rat passive cutaneous anaphylaxis (PCA) assay but also in the in vitro fragmented rat and primate lung assay.

A method for the fluorometric assay of histamine in tissues.

Gross dissection of brain showed no marked localization of brain histamine and a sensitive and specific fluorometric method for the estimation of histamine in tissues was described.

In-vitro sensitization of monkey lung fragments with human ragweed atopic serum.