Immunolocalization of retinoic acid receptors in rat, mouse and human ovary and uterus.

@article{Zhuang1994ImmunolocalizationOR,
  title={Immunolocalization of retinoic acid receptors in rat, mouse and human ovary and uterus.},
  author={Y. H. Zhuang and Timo J Ylikomi and Maria Lindfors and Sari Piippo and Pentti Tuohimaa},
  journal={The Journal of steroid biochemistry and molecular biology},
  year={1994},
  volume={48 1},
  pages={61-8}
}
We raised an antibody against a synthetic peptide corresponding to amino acids 155-174 of human retinoic acid receptor alpha (RAR-alpha). The sequence is highly homologous in all RARs and their isoforms. When mouse and human RARs (alpha, beta and gamma) expressed in Cos cell were analysed with immunoblot, all receptors gave a specific 51 K signal. Mouse RAR-gamma gave an additional signal corresponding to 58 K. In human teratocarcinoma cells (F9) both 51 and 58K molecule sizes were detected… CONTINUE READING

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