Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies

  title={Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies},
  author={Jan Woodcock-Mitchell and R Eichner and William Andrew Nelson and T T Sun},
  journal={The Journal of Cell Biology},
  pages={580 - 588}
Three monoclonal antibodies (AE1, AE2, and AE3) were prepared against human epidermal keratins and used to study keratin expression during normal epidermal differentiation. Immunofluorescence staining data suggested that the antibodies were specific for keratin-type intermediate filaments. The reactivity of these antibodies to individual human epidermal keratin polypeptides (65-67, 58, 56, and 50 kdaltons) was determined by the immunoblot technique. AE1 reacted with 56 and 50 kdalton keratins… CONTINUE READING
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Keratinization and structural organization in epidermal cell cultures

  • N. E. Fusonig, D. Breitkreutz, M. Lueder, P. Bouhamp, P.K.M. Worst
  • In International Cell Biology,
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