Immunolocalization of glucose transporter GLUT4 within human skeletal muscle.

  title={Immunolocalization of glucose transporter GLUT4 within human skeletal muscle.},
  author={Jacob E. Friedman and Ronald W Dudek and Donna S Whitehead and Deborah L. Downes and Wilhelm R. Frisell and J. Fern{\'a}ndez Caro and Gerald L Dohm},
  volume={40 1},
To investigate the cellular and subcellular distribution of glucose transporters in skeletal muscle, the glucose transporter isoform GLUT4 was localized in human muscle by electron microscopy via immunogold labeling with monoclonal (1F8) or COOH-terminal peptide polyclonal (ECU4) antibody and in isolated rat membranes by Western blot. There was no labeling of GLUT4 in endothelial cells of the capillaries. There also was no labeling of GLUT4 on the surface plasma membrane (sarcolemma) under… CONTINUE READING


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