Immunolocalization of electroneutral Na-HCO(3)(-) cotransporter in rat kidney.

  title={Immunolocalization of electroneutral Na-HCO(3)(-) cotransporter in rat kidney.},
  author={Henrik Vorum and T H Kwon and Christiaan M. Fulton and B Simonsen and Inyeong Choi and Walter F. Boron and Arvid B. Maunsbach and S\oren Lyhne Nielsen and Christian Aalkjaer},
  journal={American journal of physiology. Renal physiology},
  volume={279 5},
An electroneutral Na-HCO(3)(-) cotransporter (NBC(N)1) was recently cloned, and Northern blot analyses indicated its expression in rat kidney. In this study, we determined the cellular and subcellular localization of NBC(N)1 in the rat kidney at the light and electron microscopic level. A peptide-derived antibody was raised against the COOH-terminal amino acids of NBC(N)1. The affinity-purified antibody specifically recognized one band, approximately 180 kDa, in rat kidney membranes. Peptide-N… CONTINUE READING
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