Immunolocalization of Antifreeze Proteins in Winter Rye Leaves, Crowns, and Roots by Tissue Printing.

@article{Antikainen1996ImmunolocalizationOA,
  title={Immunolocalization of Antifreeze Proteins in Winter Rye Leaves, Crowns, and Roots by Tissue Printing.},
  author={Miia S H Antikainen and Marilyn Ford Griffith and Jiaming Zhang and W. C. Hon and DSC. Yang and Kaarina Pihakaski-Maunsbach},
  journal={Plant physiology},
  year={1996},
  volume={110 3},
  pages={845-857}
}
During cold acclimation, antifreeze proteins (AFPs) that are similar to pathogenesis-related proteins accumulate in the apoplast of winter rye (Secale cereale L. cv Musketeer) leaves. AFPs have the ability to modify the growth of ice. To elucidate the role of AFPs in the freezing process, they were assayed and immunolocalized in winter rye leaves, crowns, and roots. Each of the total soluble protein extracts from cold-acclimated rye leaves, crowns, and roots exhibited antifreeze activity… CONTINUE READING
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