Immunolocalization of 14-3-3 isoforms in brains with Pick body disease.

Abstract

Immunolocalization of 14-3-3 protein isoforms in relation to Pick bodies in Pick body disease (PBD) brains was investigated. Weakly granular immunoreactivity of 14-3-3 proteins was found in neurons in control subjects and in Pick body disease brains. In addition to this granular immunoreactivity, many Pick bodies were immunopositive for 14-3-3 proteins as confirmed with double-immunofluorescence with an anti-PHF tau (AT8) and anti-14-3-3 that recognizes all its isoforms (common). When probed with isoform-specific antibodies, Pick bodies were positive for beta, gamma, epsilon, eta, tau, and zeta isoform and exhibited immunostaining pattern similar to that observed with the anti-14-3-3 proteins (common). In addition, immunoreactivity of sigma isoform, so far considered to be exclusively extraneuronal, was unexpectedly found in Pick bodies, normal hippocampal neurons and brain homogenate from age-matched controls. Although localization of 14-3-3 proteins in Pick bodies suggests their involvement in Pick body formation, their role may be variable dependent on the isoforms differently expressed in different area in the brain.

Statistics

05001000'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

700 Citations

Semantic Scholar estimates that this publication has 700 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Umahara2004ImmunolocalizationO1, title={Immunolocalization of 14-3-3 isoforms in brains with Pick body disease.}, author={Takahiko Umahara and Toshiki Uchihara and Kuniaki Tsuchiya and Ayako Nakamura and Kenji Ikeda and Toshihiko Iwamoto and Masaru Takasaki}, journal={Neuroscience letters}, year={2004}, volume={371 2-3}, pages={215-9} }