Immunohistological demonstration of the same type of pyruvate kinase isoenzyme (M2-Pk) in tumors of chicken and rat

  title={Immunohistological demonstration of the same type of pyruvate kinase isoenzyme (M2-Pk) in tumors of chicken and rat},
  author={Manfred Reinacher and Erich Eigenbrodt},
  journal={Virchows Archiv B},
SummaryTumors of chicken and rat contain the M2-type of pyruvate kinase isoenzyme. The amount of this isoenzyme is significantly higher in malignant tumors compared with benign tumors. No alteration of the normal pyruvate kinase isoenzyme pattern was found in hyperplastic tissue. 
Purification of pyruvate kinase isoenzymes type M1 and M2 from dog (Canis familiaris) and comparison of their properties with those from chicken and rat.
Different isoelectric points and amino acid compositions have been found for the isoenzymes of lung and tumor, consistent with the assumption of a special form of pyruvate kinase M2 in dog tumor cells.
PKM2 (pyruvate kinase isoenzyme type M2)
Review on PKM2 (pyruvate kinase isoenzyme type M2), with data on DNA, on the protein encoded, and where the gene is implicated.
Pyruvate kinase type M2 and its role in tumor growth and spreading.
The switch between the tetrameric and dimeric form of M2-PK allows tumor cells to survive in environments with varying oxygen und nutrient supply.
L- and M2- pyruvate kinase expression in renal cell carcinomas and their metastases
The immunocytochemical results suggest that L-PK is a useful marker for RCC and its metastases, if acetone-fixed tissue is available, and quantitative changes of the concentration of PK and other enzymes in RCCs when compared with normal renal tissue probably reflect metabolic alterations related to tumour growth.
Immunohistological demonstration of pyruvate kinase isoenzyme type L in rat with monoclonal antibodies.
Four monoclonal antibodies (MAb) specific for the L-type isoenzyme of rat pyruvate kinase (L-PK) were produced and characterized and showed clearcut difference in L-PK content and distribution between male and female rats.
Regulation and function of pyruvate kinase M2 in cancer.
The current understanding ofPKM2 protein kinase activity and regulatory mechanisms underlying PKM2 expression, enzymatic activity, and nuclear localization are outlined, thus highlighting PKM 2 as a potential therapeutic target.
Faecal tumour M2 pyruvate kinase: a new, sensitive screening tool for colorectal cancer
The determination of Tumour M2-PK in faeces provides a new promising screening tool for colorectal tumours and TNM and Dukes' classification of the tumours revealed a strong correlation between faecal Tumours M1-PK levels and staging.
Expression of pyruvate kinase M2 in human bladder cancer and its correlation with clinical parameters and prognosis
Background Pyruvate kinase M2 (PKM2) is a key regulator of the Warburg effect and has critical functions in glycolysis, contributing to the Warburg effect, tumor growth, angiogenesis, cell division,
Pyruvate kinase type M2: a key regulator within the tumour metabolome and a tool for metabolic profiling of tumours.
  • S. Mazurek
  • Chemistry, Medicine
    Ernst Schering Foundation symposium proceedings
  • 2007
The quantification of Tumour M2-PK in EDTA plasma and stool is a tool for early detection of tumours and therapy control and is a meeting point for different oncogenes and metabolism.
Pyruvate kinase type M2 is phosphorylated at tyrosine residues in cells transformed by Rous sarcoma virus
Transformation of CECs by Rous sarcoma virus leads to a reduction in the affinity of PK for the substrate phosphoenolpyruvate, and kinetic changes were correlated with tyrosine phosphorylation of M2‐PK, but there is no direct evidence that they are caused by post‐translational modification of the enzyme.


L-alpha-alanine inhibition of pyruvate kinase from tumors of the human central nervous system.
There is ever-increasing literature pointing out that gene expression in tumour cells is different from that in normal cells from which or in which the tumour originates. In general, neoplasia is
Isozyme patterns of pyruvate kinase in various primary liver tumors induced during the process of hepatocarcinogenesis.
Both nodular hyperplasia and highly differentiated hepatomas showed total activities similar to that of normal liver, while the proportion of M2-type activity was found to increase with statistical significance.
A review of isozymes in cancer.
  • W. Criss
  • Biology, Medicine
    Cancer research
  • 1971
Summary Several distinct isozymic systems which were observed to have alterations during differentiation from the normal to the neoplastic state have been reviewed. Most of these isozymes are key
Similarities between pyruvate kinase from human placenta and tumours
The purification from human placenta of a distinct PyK isoenzyme with properties different from PyK in human liver and muscle is described and the placental isoen enzyme of PyK has a number of features in common with PyK from some human tumours.
Isozyme studies on adult, regenerating, precancerous and developing liver in relation to findings in hepatomas.
Following controlled hyperplasia and differentiation after partial hepatectomy complete liver function is restored, whereas in the precancerous liver there is a loss of control of growth and differentiation in some cells resulting in hepatoma formation, which offers a rational explanation of the diverse phenotypes observed in the transplantable Morris liver hepatomas.
Evidence for the presence of two types of pyruvate kinase in rat liver.
On the basis of comparative studies on the two types of pyruvate kinase in regenerating liver and tumor cells, the possibility that the control mechanism might depend on the stage of differentiation of the enzyme was discussed.
Isozyme variations in human malignant melanoma.
Preliminary data suggest that human malignant melanoma undergoes genetic or epigenetic changes and the alteration in some enzymes, such as 6-phosphogluconate dehydrogenase, could prove to be of diagnostic value.
Isozymes of pyruvate kinase in liver and hepatomas of the rat.
Results provide further evidence of a profound alteration of gene expression in hepatomas resulting in the loss of a specific liver-marker isozyme with loss of differentiation and its replacement in poorly differentiated hepatomas by high activities of an isozyme normally very low in the normal liver.
Isozymes of pyruvate kinase from human brain, meningiomas, and malignant gliomas.
Pyruvate kinase isozymes were studied in normal brain tissue (both fetal and adult) and in meningiomas and malignant gliomas, finding the presence of mainly K4 type and the hybrid K3M, which is in agreement with the alanine inhibition.
Activities and isoenzyme patterns of some enzymes of glucose metabolism in human primary malignant hepatoma.
The levels of a number of enzymes of carbohydrate metabolism were assayed in the cancerous and morphologically normal portions of livers from nine primary malignant hepatoma cases obtained at autopsy, showing a change from the liver type B to admixture with muscle type A.