Immunohistological and biochemical detection of fucosylated polylactosamine antigen with monoclonal antibody ACFH18 in gastric cancer and normal gastric mucosa.

@article{Dohi1990ImmunohistologicalAB,
  title={Immunohistological and biochemical detection of fucosylated polylactosamine antigen with monoclonal antibody ACFH18 in gastric cancer and normal gastric mucosa.},
  author={Taeko Dohi and Kaoru Abe and Saburo Ohshiba and Kozo Yamaguchi and Motohiko Oshima},
  journal={Japanese journal of cancer research : Gann},
  year={1990},
  volume={81 6-7},
  pages={676-81}
}
A mouse monoclonal antibody ACFH18, produced by immunizing with human gastric cell line MKN-74, recognizes a novel glycolipid antigen, fucosyl-lactoneodecaosylceramide. In the present study, we investigated the immunohistological localization of this antigen and its biochemical detection in glycolipid fraction in normal and malignant human gastric tissue, compared with other tumor-associated type-2 chain glycolipid antigens such as sialyl Lex, sialyl Lex-i and Ley. Reactivity with ACFH18 was… CONTINUE READING