Immunohistochemical study of the cytoskeleton of osteoblasts in the rat calvaria. Intermediate filaments and microfilaments as demonstrated by detergent perfusion.

Abstract

We evaluated the cytoskeleton of osteoblasts in the rat calvaria by transmission electron microscopy after perfusion with a mixture of Triton X-100 and glutaraldehyde and immunoelectron microscopy. In osteoblasts, microfilaments (5-6 nm in diameter) and intermediate filaments (10-12 nm) were arranged in networks or bundles, avoiding cell organelles such as… (More)

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