Immunohistochemical staining of cyclooxygenases with monoclonal antibodies.

  title={Immunohistochemical staining of cyclooxygenases with monoclonal antibodies.},
  author={Ghassan M. Saed},
  journal={Methods in molecular biology},
Immunohistochemistry is an important tool that is often used for the diagnosis of several diseases in the pathology laboratory. The quality and sensitivity of immunohistochemical staining is affected by formalin fixation, which results in variable loss of antigenicity, known as a masking effect. While the sensitivity of immunohistochemistry is excellent for certain antigens, other antigens such as COX-1 and COX-2 are difficult to identify, especially in formalin-fixed, paraffin sections… CONTINUE READING