Immunohistochemical localization of the murine transferrin receptor (TfR) on blood–tissue barriers using a novel anti-TfR monoclonal antibody

@article{Kissel1998ImmunohistochemicalLO,
  title={Immunohistochemical localization of the murine transferrin receptor (TfR) on blood–tissue barriers using a novel anti-TfR monoclonal antibody},
  author={Karin Kissel and Stefan Hamm and Martina Schulz and Annunciata Vecchi and Cecilia Garlanda and Britta Engelhardt},
  journal={Histochemistry and Cell Biology},
  year={1998},
  volume={110},
  pages={63-72}
}
 A novel monoclonal antibody (mAb), 8D3 (IgG2a), that specifically recognizes the murine transferrin receptor (TfR) was produced by immunizing a Lewis rat with a polyoma middle T oncogene-transformed endothelioma cell line. The 8D3 mAb was obtained by immunohistochemical screening for exclusive staining of vessels forming a blood–brain barrier (BBB), but not of other vessels. The anti-TfR mAb 8D3 recognizes the TfR also in FACS analysis and in western blots and should prove to be useful for… CONTINUE READING