Immunohistochemical localization of epidermal growth factor-like activity in the hamster ovary with a polyclonal antibody.

@article{Roy1990ImmunohistochemicalLO,
  title={Immunohistochemical localization of epidermal growth factor-like activity in the hamster ovary with a polyclonal antibody.},
  author={Shyamal K. Roy and Glenna Greenwald},
  journal={Endocrinology},
  year={1990},
  volume={126 3},
  pages={1309-17}
}
A polyclonal antibody to murine epidermal growth factor (EGF) was generated in rabbits and characterized by RIA, Western blots, and dot blotting. The antibody detected as little as 0.01 ng of mouse EGF in dot blots at 1:100,000 dilution and 5 pg of EGF in RIA at 1:50,000 dilution; it did not cross-react with transforming growth factor-alpha, insulin-like growth factor, or fibroblast growth factor. Ovarian EGF content peaked (17 +/- 2 pg/nonluteal ovary) on Day 1 (estrus as determined by copious… CONTINUE READING

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