• Biology, Medicine
  • Published in
    Investigative ophthalmology…
    1982

Immunohistochemical localization of collagen types I and II in the developing chick cornea and tibia by electron microscopy.

@article{Hendrix1982ImmunohistochemicalLO,
  title={Immunohistochemical localization of collagen types I and II in the developing chick cornea and tibia by electron microscopy.},
  author={Mary J. C. Hendrix and Elizabeth D. Hay and Klaus von der Mark and Thomas F. Linsenmayer},
  journal={Investigative ophthalmology & visual science},
  year={1982},
  volume={22 3},
  pages={
          359-75
        }
}
Monoclonal and conventional antibodies against collagen types I and II were used for immunofluorescence and immuno-electron microscopic studies of developing chick corneas (5-day-old embryos to adult) and embryonic limb cartilages. Secondary antibodies were labeled with rhodamine or ferritin. We found that the 5-day primary corneal stroma stains uniformly at the light microscope level with both monoclonal and rabbit antibodies to collagen types I and II. At the electron microscope level, the… CONTINUE READING

References

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