Immunohistochemical localization of PDE10A in the rat brain

@article{Seeger2003ImmunohistochemicalLO,
  title={Immunohistochemical localization of PDE10A in the rat brain},
  author={T. Seeger and Brenda Bartlett and T. Coskran and J. Culp and L. James and David L Krull and J. Lanfear and A. Ryan and C. J. Schmidt and C. Strick and A. Varghese and Robert D. Williams and Patricia G Wylie and F. Menniti},
  journal={Brain Research},
  year={2003},
  volume={985},
  pages={113-126}
}
PDE10A is a newly identified cAMP/cGMP phosphodiesterase for which mRNA is highly expressed in the mammalian striatum. In the present study, PDE10A protein and mRNA expression throughout the rat brain were determined, using a monoclonal antibody (24F3.F11) for Western blot and immunohistochemical analyses and an antisense riboprobe for in situ hybridization. High levels of mRNA are observed in most of the neuronal cell bodies of striatal complex (caudate n, n. accumbens and olfactory tubercle… Expand
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TLDR
The results suggest that PDE2A may modulate cortical, hippocampal and striatal networks at several levels and suggest roles in regulation of axonal excitability or transmitter release. Expand
Neuronal expression of cAMP-specific phosphodiesterase 7B mRNA in the rat brain
TLDR
The regional distribution and cellular localization of mRNA coding for the cAMP-specific phosphodiesterase 7B (PDE7B) in rat brain by in situ hybridization histochemistry offers a neuroanatomical and neurochemical base that will support the search for specific functions for cAMP dependent PDEs and for the development of specific PDE7 inhibitors. Expand
Cellular and subcellular localization of PDE10A, a striatum-enriched phosphodiesterase
TLDR
The studies indicate that PDE 10A is associated with post-synaptic membranes of the medium spiny neurons, suggesting that the specialized compartmentation of PDE10A enables the regulation of intracellular signaling from glutamatergic and dopaminergic inputs to these neurons. Expand
Immunohistochemical Localization of Phosphodiesterase 2A in Multiple Mammalian Species
  • D. Stephenson, T. Coskran, +6 authors D. Morton
  • Biology, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 2009
TLDR
It is suggested that PDE2A is evolutionarily conserved across mammalian species and support the hypothesis that the enzyme plays a fundamental role in signal transduction. Expand
Expression of the cGMP‐specific phosphodiesterases 2 and 9 in normal and Alzheimer's disease human brains
TLDR
Using radioactive in‐situ hybridization histochemistry, a widespread distribution of the mRNA for PDE2 and PDE9 was found, whereas no specific hybridization signal was observed for Pde5. Expand
Alterations in gene regulation following inhibition of the striatum-enriched phosphodiesterase, PDE10A
TLDR
Findings support the hypothesis that PDE10A modulates signal transduction in both striatal output pathways and suggest that Pde10A inhibitors may offer a unique approach to the treatment of schizophrenia. Expand
Selective Effects of PDE10A Inhibitors on Striatopallidal Neurons Require Phosphatase Inhibition by DARPP-321,2,3
TLDR
PDE10A inhibitors share with antipsychotic medications the property of activating preferentially PKA-dependent signaling in D2 MSNs, demonstrating that PDE10A regulates basal cAMP levels. Expand
Phosphodiesterase 10A Is Tethered to a Synaptic Signaling Complex in Striatum*
TLDR
PDE10A is identified as the major cAMP PDE in mouse striatum and the data indicate that phosphorylation of PDE10 has an impact on the interaction with other signaling proteins and adds an additional line of complexity to the role of Pde10 in regulation of synaptic transmission. Expand
Phosphodiesterase-10A Inverse Changes in Striatopallidal and Striatoentopeduncular Pathways of a Transgenic Mouse Model of DYT1 Dystonia
TLDR
It is reported that changes of phosphodiesterase-10A (PDE10A) can map widespread functional imbalance of basal ganglia circuits in a mouse model of DYT1 dystonia overexpressing mutant torsinA, and the inverse changes of PDE10A in striatoentopeduncular and striatopallidal projections might result over time in an imbalance between direct and indirect pathways for properly focusing movement. Expand
PDE10A and PDE10A-dependent cAMP catabolism are dysregulated oppositely in striatum and nucleus accumbens after lesion of midbrain dopamine neurons in rat: A key step in parkinsonism physiopathology
TLDR
Dopamine loss inversely regulates PDE10A gene expression in the striatum and Pde10A post-transcription in the nucleus accumbens, therein differentially modulating PDE 10A-dependent cAMP catabolism. Expand
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