Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody.

@article{Morstyn1986ImmunohistochemicalIO,
  title={Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody.},
  author={George Morstyn and K Pyke and Joy Joseph Gardner and Ruth Ashcroft and Anna de Fazio and Prithi S. Bhathal},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1986},
  volume={34},
  pages={697 - 701}
}
The ability to measure cell proliferation is important in the study of cancer biology. The usual technique for quantitating proliferating cells in tissue explant and organ culture by detection of [3H]-thymidine incorporation into DNA by autoradiography is tedious and time-consuming. We have developed a technique for identification and quantitation of bromodeoxyuridine (an analogue of thymidine) in cultured tissue explants. Fetal mouse colon explants were exposed in vitro to bromodeoxyuridine… CONTINUE READING

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