Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells.

@article{Hayashi2011ImmunohistochemicalDO,
  title={Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells.},
  author={Maki Hayashi and Shin-ichiro Inoue and Koji Takahashi and Toshinori Kinoshita},
  journal={Plant & cell physiology},
  year={2011},
  volume={52 7},
  pages={
          1238-48
        }
}
Blue light (BL) receptor phototropins activate the plasma membrane H(+)-ATPase in guard cells through phosphorylation of a penultimate threonine and subsequent binding of the 14-3-3 protein to the phosphorylated C-terminus of H⁺-ATPase, mediating stomatal opening. To date, detection of the phosphorylation level of the guard cell H⁺-ATPase has been performed biochemically using guard cell protoplasts (GCPs). However, preparation of GCPs from Arabidopsis for this purpose requires >5,000 rosette… CONTINUE READING
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