Immunohistochemical demonstration of estrogen receptors (ER) in formalin-fixed, paraffin-embedded human breast cancer tissue by use of a monoclonal antibody to ER.

@article{Andersen1988ImmunohistochemicalDO,
  title={Immunohistochemical demonstration of estrogen receptors (ER) in formalin-fixed, paraffin-embedded human breast cancer tissue by use of a monoclonal antibody to ER.},
  author={J\orn Andersen and Torben Falck Orntoft and Hans Skovgaard Poulsen},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1988},
  volume={36 12},
  pages={1553-60}
}
We describe an immunohistochemical method using a monoclonal antibody to localize estrogen receptors (ER) in formalin-fixed, paraffin-embedded tissue. The avidin-biotin-peroxidase complex method was used, preceded by trypsin treatment to expose antigenic sites. In 111 breast cancer specimens studied simultaneously by a dextran-coated charcoal (DCC) assay and the paraffin section method, agreement on receptor status was found in 101 (91%) specimens. Quantitative staining features showed a high… CONTINUE READING