Immunohistochemical Study of the Expression of MUC6 Mucin and Co-expression of Other Secreted Mucins (MUC5AC and MUC2) in Human Gastric Carcinomas

@article{Reis2000ImmunohistochemicalSO,
  title={Immunohistochemical Study of the Expression of MUC6 Mucin and Co-expression of Other Secreted Mucins (MUC5AC and MUC2) in Human Gastric Carcinomas},
  author={C. Reis and L. David and F. Carvalho and U. Mandel and C. Bol{\'o}s and E. Mirgorodskaya and H. Clausen and M. Sobrinho-Sim{\~o}es},
  journal={Journal of Histochemistry \& Cytochemistry},
  year={2000},
  volume={48},
  pages={377 - 388}
}
To investigate the expression of MUC6 mucin in gastric carcinomas, we generated a novel monoclonal antibody (MAb CLH5) using an MUC6 synthetic peptide. MAb CLH5 reacted exclusively with the MUC6 peptide and with native and deglycosylated mucin extracts from gastric tissues. MAb CLH5 immunoreactivity was observed in normal gastric mucosa restricted to pyloric glands of the antrum and mucopeptic cells of the neck zone of the body region. In a series of 104 gastric carcinomas, 31 (29.8%) were… Expand
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References

SHOWING 1-10 OF 61 REFERENCES
Expression of MUC1 and MUC2 mucins in gastric carcinomas: its relationship with the prognosis of the patients.
  • T. Utsunomiya, S. Yonezawa, +7 authors E. Sato
  • Biology, Medicine
  • Clinical cancer research : an official journal of the American Association for Cancer Research
  • 1998
TLDR
MUC1 antigen expression was associated with a poor outcome in patients with gastric cancer, irrespective of its glycosylation status, and MUC1 is thus considered to be a useful prognostic factor for poor outcomes in patients. Expand
Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody
TLDR
The finding of MUC5AC immunoreactivity in most diffuse carcinomas, which usually display morphologic and histochemical signs of gastric differentiation, suggests that all gastric carcinomas retain at least some cells with a gastric phenotype during the first steps of neoplastic development. Expand
Intestinal metaplasia of human stomach displays distinct patterns of mucin (MUC1, MUC2, MUC5AC, and MUC6) expression.
TLDR
The mucin expression profile in the different types of intestinalMetaplasia allows the identification of two patterns: one defined by decreased levels of expression of "gastric" mucins and expression of MUC2 intestinal mucin, which corresponds to type I intestinal metaplasia, and the other defined by coexpression of "Gastric mucins" (MUC1, MUC5AC, and MUC6). Expand
Correlation of the immunohistochemical reactivity of mucin peptide cores MUC1 and MUC2 with the histopathological subtype and prognosis of gastric carcinomas
The expression of MUC1 and MUC2 mucin peptide core antigens in gastric carcinomas was studied by immunohistochemistry to determine correlations with TNM stage and histo‐pathological classificationsExpand
Mucin gene expression in normal, preneoplastic, and neoplastic human gastric epithelium.
TLDR
It is concluded that human gastric epithelium has a unique mucin gene pattern, which becomes markedly altered in preneoplastic and neoplastic specimens. Expand
Expression of fully and under‐glycosylated forms of MUC1 mucin in gastric carcinoma
TLDR
M UC1 expression, namely of the SM3 cancer‐associated epitope, is significantly associated with several aspects of gastric cancer development and progression; and MUC1 expression should not be used as a prognostic marker in patients with gastric carcinoma. Expand
Aberrant expression of gland-type gastric mucin in the surface epithelium of Helicobacter pylori-infected patients.
TLDR
The type of mucin that is normally limited to mucous glands is aberrantly expressed in surface mucous cells of H. pylori-positive patients, whereas less of the surface epithelium expresses normal surface-type gastric mucin. Expand
Heterogeneity of mucin gene expression in normal and neoplastic tissues.
TLDR
In conclusion, mucin genes are independently regulated and their expression is organ- and cell type-specific, and neoplastic transformation is associated with dys-regulated expression of both membrane-bound and secreted mucin core protein epitopes and may be due to altered mucin mRNA levels and/or alter mucin glycosylation. Expand
Overexpression or ectopic expression of MUC2 is the common property of mucinous carcinomas of the colon, pancreas, breast, and ovary
TLDR
The overexpression or ectopic expression of the MUC2 gene exhibited by mucinous carcinomas of four organs indicates a common genetic lesion associated with the mucinous tumour phenotype. Expand
The MUC6 secretory mucin gene is expressed in a wide variety of epithelial tissues
TLDR
The tissue distribution of the MUC6 secretory mucin indicates that it may function to protect epithelial tissues from a wide range of substances, and is only sparsely expressed in endocervical carcinomas. Expand
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