Immunoglobulins against gp273, the ligand for sperm-egg interaction in the mollusc bivalve Unio elongatulus, are directed against charged O-linked oligosaccharide chains bearing a Lewis-like structure and interact with epitopes of the human zona pellucida.

@article{Focarelli2003ImmunoglobulinsAG,
  title={Immunoglobulins against gp273, the ligand for sperm-egg interaction in the mollusc bivalve Unio elongatulus, are directed against charged O-linked oligosaccharide chains bearing a Lewis-like structure and interact with epitopes of the human zona pellucida.},
  author={Riccardo Focarelli and Antonietta Capone and Leonardo Ermini and Francesca Del Buono and Giovanni Battista La Sala and Marcella Balasini and Floriana Rosati},
  journal={Molecular reproduction and development},
  year={2003},
  volume={64 2},
  pages={
          226-34
        }
}
In oocytes of the mollusc bivalve Unio elongatulus, gp273 is the ligand molecule for sperm-egg interaction and binding is mediated by its O-glycans. A serum raised against this protein enabled its localization in the crater region, the area of the vitelline coat where sperm recognition occurs, and showed that after cyanogen bromide fragmentation, the anti-gp273 epitope(s) was retained by a peptide where the O-glycans are localized. In this article, we utilized purified anti-gp273… CONTINUE READING