Immunoglobulin gene expression in transformed lymphoid cells.

@article{Oi1983ImmunoglobulinGE,
  title={Immunoglobulin gene expression in transformed lymphoid cells.},
  author={Vernon T. Oi and Sherie L. Morrison and Leonard A. Herzenberg and Paul Berg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 3},
  pages={825-9}
}
Myeloma, hybridoma, and thymoma cell lines have been successfully transfected for the Escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt) by using the plasmid vector pSV2-gpt. The transformed cells synthesize the bacterial enzyme 5-phospho-alpha-D-ribose-1-diphosphate:xanthine phosphoribosyltransferase (XGPRT; EC 2.4.2.22) and have been maintained in selective medium for over 4 months. Lymphoid cell lines expressing a K immunoglobulin light chain were obtained by transfecting… CONTINUE READING