Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus

@article{Ikegami2003ImmunoglobulinGE,
  title={Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus},
  author={T. Ikegami and M. Saijo and M. Niikura and M. E. Miranda and A. B. Calaor and M. Hern{\'a}ndez and D. Manalo and I. Kurane and Y. Yoshikawa and S. Morikawa},
  journal={Epidemiology and Infection},
  year={2003},
  volume={130},
  pages={533 - 539}
}
We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RΔC (amino acid (aa) 360–739), and RΔ6 (aa 451–551) and/or… Expand
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