Immunogenicity of Neisseria gonorrhoeae lipooligosaccharide epitope 2C7, widely expressed in vivo with no immunochemical similarity to human glycosphingolipids.

@article{Gulati1996ImmunogenicityON,
  title={Immunogenicity of Neisseria gonorrhoeae lipooligosaccharide epitope 2C7, widely expressed in vivo with no immunochemical similarity to human glycosphingolipids.},
  author={S. Gulati and D. McQuillen and R. Mandrell and D. Jani and P. Rice},
  journal={The Journal of infectious diseases},
  year={1996},
  volume={174 6},
  pages={
          1223-37
        }
}
Natural infection with Neisseria gonorrhoeae may elicit a substantial antibody response directed against gonococcal lipooligosaccharide. Monoclonal antibody (MAb) 2C7 recognized a gonococcal lipooligosaccharide epitope, identified the epitope directly in 94% of 68 consecutive culture-positive genital secretions, and recognized 95% of 101 randomly chosen fresh (second-passage) gonococcal isolates. The epitope was stably maintained after multiple in vitro passages and did not compete with any of… Expand
Immunization against a Saccharide Epitope Accelerates Clearance of Experimental Gonococcal Infection
TLDR
The OS epitope defined by mAb 2C7 may represent an effective vaccine target against gonorrhea, which is rapidly becoming incurable with currently available antibiotics. Expand
Characterization of a peptide vaccine candidate mimicking an oligosaccharide epitope of Neisseria gonorrhoeae and resultant immune responses and function.
TLDR
Data indicate that a peptide can mimic an oligosaccharide epitope and may form the basis for the development of a vaccine candidate for human immunization against N. gonorrhoeae. Expand
Experimental immunization with a monoclonal anti-idiotope antibody that mimics the Neisseria gonorrhoeae lipooligosaccharide epitope 2C7.
TLDR
MAb CA1 acts as a molecular surrogate (Ab2beta) for the nominal LOS antigen and may form the basis for vaccine candidates for human immunization against Neisseria gonorrhoeae. Expand
Preclinical Efficacy of a Lipooligosaccharide Peptide Mimic Candidate Gonococcal Vaccine
TLDR
A gonococcal vaccine candidate that can be scaled up and produced economically to a high degree of purity and elicits bactericidal antibodies and is efficacious in a preclinical experimental infection model is developed. Expand
Structural and Immunochemical Characterization of aNeisseria gonorrhoeae Epitope Defined by a Monoclonal Antibody 2C7; the Antibody Recognizes a Conserved Epitope on Specific Lipo-oligosaccharides in Spite of the Presence of Human Carbohydrate Epitopes*
TLDR
This study analyzed the structure of gonococcal strain WG LOS containing the 2C7 epitope and investigated the structural requirements for expression of the epitope, showing that the conserved epitope defined by mAb2C7 could potentially be used as a safe site for the development of a vaccine candidate. Expand
Phase-Variable Heptose I Glycan Extensions Modulate Efficacy of 2C7 Vaccine Antibody Directed against Neisseria gonorrhoeae Lipooligosaccharide
Neisseria gonorrhoeae, the causative agent of the sexually transmitted infection, gonorrhea, has developed resistance to most conventional antibiotics. Safe and effective vaccines against gonorrheaExpand
Strategies for Mimicking Neisserial Saccharide Epitopes as Vaccines
TLDR
Two different strategies to mimic the 2C7 OS epitope elicited antibody responses in mice and rabbits that were bactericidal in vitro against gonococci and may form a basis for the development of vaccine candidates for human immunization against N. gonorrhoeae. Expand
The Oligosaccharide of Gonococcal Lipooligosaccharide Contains Several Epitopes That Are Recognized by Human Antibodies
TLDR
Results show that neisserial LOS contains several epitopes within its OS moiety that are recognized by human antibodies, and these epitopes are located at the reducing end of the carbohydrate moiety of each LPS. Expand
Targeting Lipooligosaccharide (LOS) for a Gonococcal Vaccine
TLDR
2C7 vaccine satisfies criteria that are desirable in a gonococcal vaccine candidate: broad representation of the antigenic target, service as a virulence determinant that is also critical for organism survival in vivo and elicitation of broadly cross-reactive IgG bactericidal antibodies when used as an immunogen. Expand
Western Blot Analysis of Neisserial Lipooligosaccharide at the Lower Femtomole Level with Normal Human Sera
TLDR
The results obtained here indicate that the binding of IgG in NHS to the LOS and LPS samples is probably due to their carbohydrate moieties, and should help not only to further characterize anti-LOS antibodies in blood and body fluids but also to analyze carbohydrate structures that are recognized by them. Expand
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Experimental immunization with a monoclonal anti-idiotope antibody that mimics the Neisseria gonorrhoeae lipooligosaccharide epitope 2C7.
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MAb CA1 acts as a molecular surrogate (Ab2beta) for the nominal LOS antigen and may form the basis for vaccine candidates for human immunization against Neisseria gonorrhoeae. Expand
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