Immunogenetic analysis of a panel of monoclonal IgG and IgM anti-PDC-E2/X antibodies derived from patients with primary biliary cirrhosis.

@article{Thomson1998ImmunogeneticAO,
  title={Immunogenetic analysis of a panel of monoclonal IgG and IgM anti-PDC-E2/X antibodies derived from patients with primary biliary cirrhosis.},
  author={R. K. Thomson and Zadie A Davis and Jeremy M. Palmer and Michael J. P. Arthur and Stephen J. Yeaman and Caroline J. Chapman and Myfanwy B Spellerberg and Freda K Stevenson},
  journal={Journal of hepatology},
  year={1998},
  volume={28 4},
  pages={
          582-94
        }
}
BACKGROUND/AIMS Autoantibodies with specificity for the E2 component of the pyruvate dehydrogenase complex (PDC-E2) are commonly present in primary biliary cirrhosis. The aim of this study was to generate and characterise human anti-PDC-E2 monoclonal antibodies and analyse immunoglobulin gene usage and mutation for clues to pathogenesis. METHODS Peripheral B-lymphocytes from two patients with primary biliary cirrhosis were used to generate heterohybridomas secreting PDC-E2 specific monoclonal… CONTINUE READING