Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS.

@article{Liu2005ImmunofluorescenceAF,
  title={Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS.},
  author={I-Jung Liu and Pei-Jer Chen and Shiou-Hwei Yeh and Y Chiang and L M Huang and M Chang and S. Chen and Pan-Chyr Yang and S -S Chang and W Wang},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 5},
  pages={2444-8}
}
An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10… CONTINUE READING