Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers.


An immunofluorescence assay was developed to identify proteins specifically binding to oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-kappaB p50 protein was incubated with either bead-bound NF-kappaB consensus sequence or a bead-bound ODN combinatorial library and adsorption was then assessed using a… (More)


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@article{Yang2003ImmunofluorescenceAA, title={Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers.}, author={Xianbin Yang and Xin Li and Tarl W. Prow and Lisa M. Reece and Suzanne E Bassett and Bruce A. Luxon and Norbert K. Herzog and Judy F Aronson and Robert E. Shope and James F. Leary and David G. Gorenstein}, journal={Nucleic acids research}, year={2003}, volume={31 10}, pages={e54} }