Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens.

@article{Burgio1984ImmunofluorescenceAE,
  title={Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens.},
  author={Vito Lelio Burgio and Albarlo Martini and Maria Antonietta Avanzini and Marco Paulli and Rosario Rosso},
  journal={Applied pathology},
  year={1984},
  volume={2 3},
  pages={128-34}
}
We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-micron-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: (1) reduction of background fluorescence, (2) higher resolution of morphologic details, (3) possibility of studying undecalcified bone marrow specimens… CONTINUE READING
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