Immunoflourescent staining of cytoplasmic and spindle microtubules in mouse fibroblasts with antibody to r protein ( tubulin / microtubule accessory proteins / networks

@inproceedings{ConnollyImmunoflourescentSO,
  title={Immunoflourescent staining of cytoplasmic and spindle microtubules in mouse fibroblasts with antibody to r protein ( tubulin / microtubule accessory proteins / networks},
  author={Joe Connolly and Vitauts I. Kalnins and DON W. CLEVELANDt and MARC W. KIRSCHNERt}
}
T protein isolated from porcine brain microtubules was further purified by electrophoretic elution from polyacrylamide gels and used to prepare antisera in rabbits. The antiserum to T specifically stains mitotic spindles and a filamentous network within mouse fibroblasts when the indirect immunofluorescence technique is used. The staining of the filamentous… CONTINUE READING