Immunofiltration purification for urinary leukotriene E4 quantitation.

  title={Immunofiltration purification for urinary leukotriene E4 quantitation.},
  author={Jay Y. Westcott and Sheldon Sloan and S E Wenzel},
  journal={Analytical biochemistry},
  volume={248 2},
Leukotriene E4 (LTE4) is a major leukotriene metabolite in urine. Urinary LTE4 concentration is often utilized as an index of total leukotriene synthesis. A novel method employing immunofiltration for the purification of urinary LTE4 was developed. This immunofiltration method is based upon the addition of excess anti-LTE4 antibody to urine which binds LTE4. Separation of bound LTE4 (high M(r)) from high levels of unbound contaminants (low M(r)) is then accomplished by filtration through a 10… CONTINUE READING