Immunoenzymatic quantitative analysis of antigens expressed on the cell surface (cell-ELISA).

@article{Loureno2010ImmunoenzymaticQA,
  title={Immunoenzymatic quantitative analysis of antigens expressed on the cell surface (cell-ELISA).},
  author={Elaine Vicente Lourenço and Maria-Cristina Roque-Barreira},
  journal={Methods in molecular biology},
  year={2010},
  volume={588},
  pages={301-9}
}
Cell-enzyme-linked immunosorbent assay (cell-ELISA) is an useful technique for the quantitative analysis of cell surface antigen expression that was developed on the basis of enzyme immunohistochemistry (EIH) and ELISA. Since its development, which was made possible by the establishment of monoclonal antibody technology, a wide range of cell types and surface molecules were analyzed by cell-ELISA. Here we show four variants of this method and provide a brief comparison of cell-ELISA with flow… CONTINUE READING

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