Immunoelectron microscopical visualization of ribonucleoproteins in the chromatoid body of mouse spermatids.

@article{Biggiogera1990ImmunoelectronMV,
  title={Immunoelectron microscopical visualization of ribonucleoproteins in the chromatoid body of mouse spermatids.},
  author={Marco Biggiogera and Stanislav Fakan and Gunilla Leser and Terence E. Martin and Julian A. Gordon},
  journal={Molecular reproduction and development},
  year={1990},
  volume={26 2},
  pages={
          150-8
        }
}
The chromatoid body (CB), a cytoplasmic organelle present only in germ cell line, was studied at the electron microscopic level in mouse spermatids using cytochemical techniques and specific antibodies directed against sn-RNPs, hnRNPs, and ribosomal proteins. We found that specific staining for DNA as well as the use of monoclonal anti-DNA antibodies show a complete absence of DNA in the CB. The CB remains stained, however, after the application of the ethidium bromide-PTA technique, suggesting… CONTINUE READING
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