Immunoelectron microscopic localization of variable proteins on the surface of Mycoplasma bovis.

@article{Behrens1996ImmunoelectronML,
  title={Immunoelectron microscopic localization of variable proteins on the surface of Mycoplasma bovis.},
  author={Alexander Behrens and Martin Heller and Ricardo Francisco Rosenbusch and Helga Kirchhoff},
  journal={Microbiology},
  year={1996},
  volume={142 ( Pt 7)},
  pages={1863-71}
}
The ultrastructural distribution and immunological accessibility of the variable surface proteins VspA, VspB, VspC and VspD were determined by immunoelectron microscopy on the surface of negatively stained cells of Mycoplasma bovis PG45 and 18 subclones, expressing either one or two of the Vsps. The variable proteins VspA, VspB, VspC and VspD, recognized by two monoclonal antibodies (mAb 1E5 and mAb 87-2) and visualized by goat-anti-murine-lgM labelled with gold particles, showed identical… CONTINUE READING

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