Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase.

  title={Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase.},
  author={Silvana Carnevale and Marta Rodr{\'i}guez and Eduardo Alfredo Guarnera and Carlos Garcia Carmona and Tamara B Tanos and Sergio Oscar Angel},
  journal={Diagnostic microbiology and infectious disease},
  volume={41 1-2},
Cathepsin L1, a cysteine protease secreted by the gastrodermis of juvenile and adult Fasciola hepatica, was expressed in Escherichia coli as a fusion protein containing the proregion, supplied with six histidyl residues at the N-terminal end (rproCL1). In this study we tested its potential as antigen for the serologic diagnosis of F. hepatica infections by enzyme-linked immunosorbent assay (ELISA). The analyzed human sera included 16 positive samples, 99 negative controls and 111 from… CONTINUE READING